Microchannel Immunoassays with Electrochemical Detection in Devices Constructed from Low Temperature Co-fired Ceramic and Screen Printed Gold

We report a self-contained electrochemical enzyme linked immunosorbent assay in microchannel devices constructed from low temperature co-fired ceramic with screen-printed gold for fast and sensitive assays. No one has previously reported a device constructed from these materials for combined immobilization and electrochemical detection. These low temperature co-fired ceramic microchannel devices used for electrochemical immunoassay contain four integrated, individuallyaddressable screen-printed gold electrodes. The total channel volume is under 0.8 μl. The entire immunoassembly and electrochemical detection is carried out within the microchannel device-using mouse IgG as the model analyte and electrochemical detection of paminophenol is possible within 15 s of introducing the enzyme substrate, p-aminophenyl phosphate into the microchannel ELISA.